Effects of tensile strain and Wnt3A, separately or combined, on chondrocyte early response gene transcription. Untreated chondrocytes served as controls. (A) c-fos, (B) c-jun and (C) Lef1 gene expression levels analysed immediately or (D), (E) and (F) following a four-hour recovery period respectively. Gene expression levels were assessed using quantitative PCR, normalised to the housekeeping gene 18s and relative to the untreated cells. Data are presented as mean ± S.E.M. (n = 6; *P < 0.05, **P < 0.01, ***P < 0.001).