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Table 1 Primer sequences of bovine genes of interest

From: Effects of Wnt3A and mechanical load on cartilage chondrocyte homeostasis

Gene name

Primer sequence

Tm (°C)

Product size (bp)

Lef1

For 5'-tcagcctgtgtatcccatca-3'

Rev 5'-tgaggcttcacgtgcattag-3'

60

219

c-fos

For 5'-cggctttgcagacagagattg-3'

Rev 5'-gggtgaaggcctcctcagatt-3'

60

192

c-jun

For 5'-taaactaagcccacgcgaag-3'

Rev 5'-ctcagactggaggaacgagg-3'

60

100