Figure 4From: Regenerative potential of human muscle stem cells in chronic inflammation Telomere length of human satellite cells. Telomere length was measured by flow cytometry using fluorescence in situ hybridization without (A) or with the fluorescein-conjugated PNA probe (B). Propidium iodide (PI) was used for labeling DNA content. The telomere probe signal was measured in the FL-1 channel and the PI signal in the FL-3 channel. G0/1 phase sample and control cells were gated according to their PI specific signal on a dot blot (FL1-H vs. FL3-H). Relative telomere length (RTL) was calculated by: RTL = (mean FL1 sample cells with probe - mean FL1 sample cells without probe)/(mean FL1 control cells with probe - mean FL1 control cells without probe) * 2 * 100%. Representative images are shown of the one of the RA patients. For each sample analyzed 20,000 counts were acquired.Back to article page