Figure 1

Gating and control rationale for CD20⁺ T-cell selection. (A) (i) Typical flow cytometry dot plot of the forward and side scatter of peripheral blood mononuclear cells (PBMCs). (ii) Dot plot of P1 gated cells (as indicated in A) stained with CD20-FITC and CD19-PE and shown in 'R2'. (iii) Dot plot of P1 gated cells, except 'R2', stained with CD20-FITC and CD3-CyQ. (B) (i) Typical flow cytometry dot plot of the forward and side scatter of PBMCs. (ii) Dot plot of P1 gated cells stained with isotype control IgG-FITC and isotype control IgG-PE and shown in 'R2' (iii). Dot plot of P1 gated cells, except 'R2', stained with isotype control IgG-FITC and isotype control CD3-CyQ. (C) (i) Typical flow cytometry dot plot of the forward and side scatter of PBMCs. (ii) Dot plot of P1 gated cells stained with CD20-FITC and CD19-PE post-RTX treatment and shown in 'R2'. (iii) Dot plot of P1 gated cells, except 'R2', stained with CD20-FITC and CD3-CyQ post-RTX incubation.