Notochordal cells protect nucleus pulposus cells from degradation and apoptosis: implications for the mechanisms of intervertebral disc degeneration

Table 1 Real-time RT-PCR Primers, annealing temperatures and Accession Numbers of genes evaluated

Gene	Primer Sequence	Annealing Temp °C	Accession Number
Aggrecan F	CCTGAACGACAAGACCATCGA	58	U76615
Aggrecan R	TGGCAAAGAAGTTGTCAGGCT	58
Collagen 2 F	AAGAAGGCTCTGCTCATCCAGG	58	X02420
Collagen 2 R	TAGTCTTGCCCCACTTACCGGT	58
MMP3 F	CACTCAACCGAACGTGAAGCT	58	AF135232
MMP3 R	CGTACAGGAACTGAATGCCGT	58
TIMP1 F	TCCCTGGAACAGCATGAGTTC	58	AF144763
TIMP1 R	TGTCGCTCTGCAGTTTGCA	58
ADAMTS4 F	CCTGGCAACGAGGACTCAAC	58	NM_181667.1
ADAMTS4 R	GGGTAAACAGAATGGCTGTGTCA	58
CD44 F	CGGGTTCATAGAAGGGCATGT	58	X62881.1
CD44 R	TTGTTCGCAGCACAGATGGA	58
Link protein F	AAGCTGACCTACGACGAAGCG	58	NM_174288.1
Link protein R	CGCAACGGTCATATCCCAGA	58
IL-6 F	CTGGGTTCAATCAGGCGAT	58	NM_173923.2
IL-6 R	CAGCAGGTCAGTGTTTGTGG
ANKH F ANKH R	TCGGTGTGGACTTTGCCTTT ACTGGAACCGGGAAGAAGGA	58	NP_001103263

ISSN: 1478-6362