The D34A mutation of Unc93B1 up-regulates TLR7 response and down-regulates TLR9 response. (A and B). Empty vector was transfected to bone marrow derived stem cells (BMSCs) from wild tipe mice (gray bars). Empty vector (yellow bars), wild type Unc93B1 expressing vector (blue bars), or D34A Unc93B1 expressing vector (red bars) were transfected to BMSCs from 3d mice. Transfected BMSCs were cultured with puromycin and GM-CSF to differentiate to dendritic cells (DCs). After differentiation, DCs were harvested and stimulated by TLR7 ligands (A, loxoribine, μg/ml) or TLR9 ligands (B, CpG-B, nM). Culture supernatant was corrected and subjected to ELISA for measurement of IL-12p40 (ng/ml).