T cells and B cells are activated in Unc93b1D34A/D34A mice. (A or D) Flow cytometry analysis for memory T cells (A upper, or D) or Th1/Th17 cells (A lower). (B) Expression of ICOS was measured by cell surface staining of CD4+ T cells. Mean fluorescent intensity (MFI) was calculated and indicated by dots. Black bars in the graph indicate average of MFI. (C) Flow cytometry analysis for matrginal zone B cells. (E) Survival curves of Unc93b1D34A/D34A (Red) or Unc93b1D34A/D34AIghm-/- (Green) mice.