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Table 2 Articular cartilage histologic evaluation criteria

From: Effect of in uteroexposure to diethylstilbestrol on lumbar and femoral bone, articular cartilage, and the intervertebral disc in male and female adult mice progeny with and without swimming exercise

Criteria for histologic evaluation of articular cartilage
Safranin O-fast green staining
0 No loss
1 Mild loss
2 Moderate loss
3 Severe loss
Chondrocyte loss
0 No decrease in cells
1 Minimal decrease in cells
2 Moderate decrease in cells
3 Marked decrease in cells
4 Very extensive decrease in cells
Structure
0 Normal
1 Surface irregularities
2 One to three superficial clefts
3 More than three superficial clefts
4 One to three clefts extending into the middle zone
5 More than three clefts extending into the middle zone
6 One to three clefts extending into the deep zone
7 More than three clefts extending into the deep zone
8 Clefts extending to calcified cartilage
Chondrocyte clones
0 Normal
1 Minimal (≤4)
2 Moderate (> 4 but ≤8)
3 Marked (> 8)
Tidemark integrity
0 Intact
1 Crossed by blood vessels
Tidemark duplication
0 No change
1 Duplication of tidemark
2 No visible tidemark remaining
Subchondral bone thickness
0 Subchondral bone thickness less than thickness of cartilage
1 Subchondral bone thickness equal to thickness of cartilage
2 Subchondral bone thickness more than thickness of cartilage