Generation and visualization of neutrophil extracellular traps (NETs) from myeloid cell lines and human primary neutrophils. A. Three cell line models used to generate neutrophils and NETs. Murine cell lines EPRO and MPRO were induced with ATRA and GM-CSF to differentiate into neutrophils. EPRO cells were generated by differentiating the EML multipotent progenitor cell line with ATRA, GM-CSF and IL-3. NETs were generated by stimulating cell line-derived or primary human neutrophils with hydrogen peroxide. B. Fluorescent (DAPI) images of NETs produced from neutrophils derived from EPRO, HL-60 or MPRO cell lines, or from primary human neutrophils. Color channels are inverted to yield white background for improved contrast. 400× magnification. ATRA, all-trans retinoic acid; DAPI, 4',6-diamidino-2-phenylindole; DMSO, dimethyl sulfoxide; GM-CSF, granulocyte macrophage colony-stimulating factor; IL-3, interleukin-3.