MKK7 ASOs selectively reduce MKK7 mRNA and protein levels in normal C57BL/6 mice. Three 2'-O-Methoxyethyl chimeric ASOs for MKK7 or control ASO were injected i.v. at the indicated doses and ankle joints, liver, and spleen were collected three days after injection of ASO. (A) Total mRNA was prepared from ankle joints and MKK7 mRNA expression was analyzed by quantitative PCR (Values = Mean ± SE, n = 3 mice for each group, *P < 0.05 vs. control ASO). (B) Western blot analysis of proteins in ankle lysates derived from naive and control or MKK7 ASO (50 mg/kg) injected mice. MKK7 ASOs decreased MKK7 protein levels but did not decrease MKK3, MKK4, and MKK6 levels. (C) MKK7 ASOs decreased MKK7 mRNA levels in liver and spleen (Values = Mean ± SE, n = 3 mice for each group, *P < 0.05 vs. control ASO).