Local delivery of AAV2-CTLA4IgG decreases sialadenitis and improves gland function in the C57BL/6.NOD-Aec1Aec2mouse model of Sjögren's syndrome

Table 1 Cytokine production local and systemic immune system in vector treated mice (pg/ml)

Serum, splenocytes, and salivary gland DLNs were collected at the end of the study. Splenocytes and DLN cells were pooled according to vector treatment group. Culture supernatants were collected following incubation with or without ConA for 48 h. Cultures and serum were then analyzed for levels of the indicated cytokines (in pg/ml) by multi-cytokine assay in duplicate. For cell cultures, values are the mean of duplicate tests of ConA treated cells subtracted from media alone. For serum, the data shown were the median ± SD of each group of mice (AAV2-LacZ [n = 6] and AAV2-CTLA4IgG [n = 7], respectively]. Mann-Whitney U test was used to analyze differences of cytokine production in serum. ↑: Production of cytokines in the AAV2-CTLA4IgG group is ≥ 50% higher than in AAV2-LacZ group; ↓: Production of cytokines in the AAV2-CTLA4IgG group is ≥ 50% lower than the AAV2-LacZ group.

ISSN: 1478-6362