Figure 3From: Altered responsiveness to TGF-β results in reduced Papss2 expression and alterations in the biomechanical properties of mouse articular cartilageBovine articular chondrocytes in micromass culture. Bovine articular chondrocytes grown in micromass cultures were left untreated (A) and (C) or treated with transforming growth factor β (TGF-β) (B) and (D) for 7 days. Cultures were stained for Alcian blue (A) and (B) or alkaline phosphatase (C) and (D). Quantification of staining showed an increase in Alcian blue staining (E) and a decrease in alkaline phosphatase activity after treatment with TGF-β (F). *P-value < 0.05 by t-test. pNP: p-nitophenol.Back to article page