Effect of IL-1β on pro (A) and anti-angiogenic (B) factor gene expression. FSC were cultured for 5 or 24 h in the absence or in the presence of IL-1β (1 ng/ml). Total RNA was isolated in cellular extract and pro-angiogenic (VEGF, bFGF, NGF, Ang-1 and MMP-2) (A) and anti-angiogenic (TSP-1, TSP-2 and VEGI) (B) mRNA expression was analysed by real time RT PCR. Results are expressed as a percentage of the basal condition (cells incubated without IL-1β). Each condition was tested in duplicate. Results are expressed as mean ± SEM of three independent experiments.