Effect of CS on IL-1β-dependent anti-angiogenic gene expression. SFC were pre-incubated with CS (10, 50, 200 μg/ml) for 1 h. IL-1β (1 ng/ml) was added to culture medium for 5 or 24 h incubation. Total RNA was isolated in SFC and VEGI (A), TSP-1 (B) and TSP-2 (C) mRNA expression was analyzed by real time RT PCR. Results are expressed as a percentage of the control (cells incubated without CS). Each condition was tested in duplicate. Results are expressed as mean ± SD of five independent experiments. Comparison of mean values was performed by Mann-Whitney non-parametric analysis. ***P < 0.001: CS treated group was significantly different from control.