Cytokines profiling by multiplex analysis in experimental arthritis: which pathophysiological relevance for articular versus systemic mediators?

Table 2 Histological grading of knee synovium in rats developing antigen-induced arthritis

Time point	Synovial lining hyperplasia		Fibrosis		Proliferative blood vessels		Diffuse infiltrates		Perivascular infiltrates
	Sensitized	Saline-injected	Sensitized	Saline-injected	Sensitized	Saline-injected	Sensitized	Saline-injected	Sensitized	Saline-injected
Day 0	0.2 ± 0.2	0.2 ± 0.2	0.6 ± 0.2	0.6 ± 0.3	0.6 ± 0.2	0.6 ± 0.3	0.4 ± 0.3	0.4 ± 0.3	0.0 ± 0.0	0.0 ± 0.0
7 hours	1.2 ± 0.4	0.2 ± 0.2	0.8 ± 0.4	0.6 ± 0.3	0.8 ± 0.2	0.8 ± 0.2	1.2^a ± 0.2	0.8 ± 0.4	2.8^a ± 0.6	1.6^a ± 0.6
Day 1	1.6^a ± 0.4	0.8 ± 0.2	1.6^a ± 0.4	0.6 ± 0.3	0.6 ± 0.2	0.4 ± 0.2	2.2^a ± 0.4	1.0 ± 0.0	2.0^a ± 0.5	1.8^a ± 0.4
Day 2	2^a ± 0.3	0.2 ± 0.2	2.4^a ± 0.3	0.6 ± 0.3	1.0 ± 0.0	0.6 ± 0.4	3.4^a ± 0.3	0.4 ± 0.3	2.6^a ± 0.5	0.4 ± 0.3
Day 3	3.2^a ± 0.6	0.4 ± 0.3	2.2^a ± 0.5	0.6 ± 0.3	1.8^a ± 0.2	0.8 ± 0.4	3.4^a ± 0.4	0.4 ± 0.3	2.0^a ± 0.5	0.4 ± 0.3
Day 7	2.5^a ± 0.7	0.3 ± 0.3	3.5^a ± 0.3	0.3 ± 0.3	2.5^a ± 0.5	0.5 ± 0.3	2.8^a ± 0.6	0.3 ± 0.4	1.8^a ± 0.5	0.4 ± 0.4
Day 14	3.3^a ± 0.5	0.0 ± 0.0	4.0^a ± 0.0	0.3 ± 0.3	1.8 ± 0.5	1.0 ± 0.4	2.0^a ± 0.0	0.3 ± 0.3	1.8^a ± 0.3	0.8 ± 0.3

Rats were immunized subcutaneously with 0.5 g of methylated bovine serum albumin (mBSA) emulsified with complete Freund's adjuvant 21 and 14 days prior to articular injection (day 0) of 0.5 mg of mBSA (sensitized) or 50 μL of saline (contralateral) into the knee joints. Values are expressed as the mean ± standard error of the mean of five rats per time. ^aP < 0.05 in comparison with day 0.

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