Skip to main content

Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Figure 8 | Arthritis Research & Therapy

Figure 8

From: TNFα modulates protein degradation pathways in rheumatoid arthritis synovial fibroblasts

Figure 8

Rheumatoid arthritis synovial fibroblasts are more resistant to proteasome and autophagy/lysosome inhibitors than other fibroblasts. Fibroblasts (n = 6 rheumatoid arthritis (RA), n = 3 osteoarthritis, n = 3 dermal), stimulated or not with 10 ng/ml TNFα, were cultured with (a) 2 μg/ml tunicamycin, (b) 0.5 μM MG132, (c) 4 mM 3-methyladenine (3-MA) or (d) 12.5 μM chloroquine (CQ) for 72 hours. An XTT assay was performed and the survival of the cells was determined. Bars show standard error of the mean. Significant differences in control fibroblasts compared with RA synovial fibroblasts or the response of TNFα-stimulated cultures compared with non-induced cultures: *P < 0.05, **P < 0.01.

Back to article page