Table 1 Oligonucleotide primers used in real-time PCR assay
Gene | Forward primer sequence | Reverse primer sequence |
|---|---|---|
BMP-2 | CCCAGCGTGAAAAGAGAGAC | GGAAGCAGCAACGCTAGAAG |
BMPR-IA | TTTATGGCACCCAAGGAAAG | TGGTATTCAAGGGCACATCA |
LEF-1 | CCCTTCCAACTCTCCTTTCC | TTGAAGGGGATCATCTCGTC |
Osteocalcin | AGAGTCCAGCAAAGGTGCAG | TCAGCCAACTCGTCACAGTC |
LRP-5 | GTGCCTGGGTGAGATTCTTC | CACGAAGACTGCGAAACAGA |
MMP-7 | TGCTCACTTCGATGAGGATG | TGGGGATCTCCATTTCCATA |
MMP-13 | TGGCATTGCTGACATCATGA | GCCAGAGGGCCCATCAA |
MMP-14 | GAGCTCAGGGCAGTGGATAG | GGTAGCCCGGTTCTACCTTC |
MMP-9 | TTGACAGCGACAAGAAGTGG | GCCATTCACGTCGTCCTTAT |
BMP-4 | ATGAAGCCCCCAGCAGAAGT | TCACATTGTGGTGGACCAGTC |
ADAMTS-4 | TCCTGCAACACTGAGGACTG | GGTGAGTTTGCACTGGTCCT |
ADAMTS-5 | CAGCAGTGCAACCTGACATT | CAGATTCTCCCCTTTCCACA |
COL10A1 | CAGGCATAAAAGGCCCAC | GTGGACCAGGAGTACCTTGC |
COL2A1 | ATGACAATCTGGCTCCCAACACTGC | GACCGGCCCTATGTCCACACCGAAT |
Aggrecan | TGAGGAGGGCTGGAACAAGTACC | GGAGGTGGTAATTGCAGGGAACA |
GAPDH | GAGTCAACGGATTTGGTCGT | GACAAGCTTCCCGTTCTCAG |