Serum and cytosolic macrophage migration inhibitory factor levels in peripheral blood mononuclear cells. (A) Serum macrophage migration inhibitory factor (MIF) levels determined by ELISA. (B) Intracellular MIF levels determined by western blotting and quantified with installed density-analysis software. The value for each individual is represented as a single point. Horizontal lines represent the median values for each group. Wilcoxon signed-ranks test used to analyze any two groups. Ctrl, healthy donors; SR, steroid-resistant group; SS, steroid-sensitive group.