Efficacy of an immunotoxin to folate receptor beta in the intra-articular treatment of antigen-induced arthritis

Table 1 Distribution of FRβ in normal rat tissues analyzed using flow cytometry or immunohistochemistry

Flow Cytometric Analysis	FRβ	CD11 b/c
TGC-elicited Peritoneal mϕ	87 ± 3.1%	95 ± 2.5%
Immunohistochemical Analysis	FRβ	CD68
Spleen
White pulp	-	++
Red pulp mϕ	+	+++
Lymph node
Cortex, tingible body mϕ	-	++
Paracortical mϕ	-	+
Medullary mϕ	-	+
Subcapsular sinus mϕ	-	+
Brain mϕ	-	+
Liver mϕ (Kupffer cells)	+	++
Lung
Perivascular/peribronchial mϕ	-	+
Alveolar mϕ	-	+
Kidney
Mesangial mϕ	-	+
Heart
Myocardial mϕ	-	+
Endocardial mϕ	-	+
Colon
Mucosa
Epithelial mϕ	-	+
Lamina propria mϕ	+	+
Muscularis mucosa mϕ	-	+
Submucosal mϕ	-	+
Muscularis externa mϕ	-	+

Frequency of cell staining (-, <100/10 fields), (+, 100-200/10 fields), and (++, 200-300/10 fields), (+++, >300/10 fields). Data was obtained from three normal rat tissues with similar results. FRβ, folate receptor β; TGC, thioglycollate.

ISSN: 1478-6362