Skip to main content

Table 1 Distribution of FRβ in normal rat tissues analyzed using flow cytometry or immunohistochemistry

From: Efficacy of an immunotoxin to folate receptor beta in the intra-articular treatment of antigen-induced arthritis

Flow Cytometric Analysis FRβ CD11 b/c
TGC-elicited Peritoneal mϕ 87 ± 3.1% 95 ± 2.5%
Immunohistochemical Analysis FRβ CD68
Spleen   
   White pulp - ++
   Red pulp mϕ + +++
Lymph node   
   Cortex, tingible body mϕ - ++
   Paracortical mϕ - +
   Medullary mϕ - +
   Subcapsular sinus mϕ - +
Brain - +
Liver mϕ (Kupffer cells) + ++
Lung   
   Perivascular/peribronchial mϕ - +
   Alveolar mϕ - +
Kidney   
   Mesangial mϕ - +
Heart   
   Myocardial mϕ - +
   Endocardial mϕ - +
Colon   
   Mucosa   
   Epithelial mϕ - +
   Lamina propria mϕ + +
   Muscularis mucosa mϕ - +
   Submucosal mϕ - +
   Muscularis externa mϕ - +
  1. Frequency of cell staining (-, <100/10 fields), (+, 100-200/10 fields), and (++, 200-300/10 fields), (+++, >300/10 fields). Data was obtained from three normal rat tissues with similar results. FRβ, folate receptor β; TGC, thioglycollate.