Figure 4

Induction of macrophage IL-10 production by T cells from synovial membranes in rheumatoid arthritis (RA-Ts) is PI3K- and p70S6K-dependent. RA-Ts were co-cultured with M-CSF-primed macrophages in the presence or absence of the PI3K inhibitor wortmannin (50 nM) or the p70S6K inhibitor rapamycin (1 nM). Bars show mean IL-10 in triplicate culture supernatants ± SD, for a representative of N = 3 replicate experiments. p70S6K = p70 S6-kinase; PI3K = phosphatidylinositol 3-kinase; Rapa = rapamycin; T:M = ratio of RA-Ts:macrophages; Wort = wortmannin. ^*P < 0.05, ^**P < 0.01.