Figure 6

Modulation by IL-1β and TNF of clock proteins in synovial fibroblasts. Abbreviations see legend to Figure 1. Box plots give the 10^th, 25^th, 50^th (median), 75^th and 90^th percentiles. A) Mean of mRNA of Period 1 and Period 2 and dose-dependent influence of IL-1β and TNF (both ng/ml) on MH7A synovial fibroblasts. Effects on CLOCK and BMAL-1 are demonstrated in Additional file 2 Figure S2. The Box Plots are generated from at least 12 independent experiments over 36 hours (n = 19 for IL-1β; n = 12 for TNF). *P < 0.05, #P < 0.005, ##P < 0.001 vs. highest concentration. B) Influence of IL-1β and TNF (both 1 ng/ml) on elapsed time to first BMAL-1 mRNA peak after synchronization. Every symbol represents the time to first peak of one patient during 72 hours of observation (sample interval two hours). *P < 0.05, ##P < 0.001 vs. control (Co).