Modulation by IL-1β and TNF of clock proteins in synovial fibroblasts. Abbreviations see legend to Figure 1. Box plots give the 10th, 25th, 50th (median), 75th and 90th percentiles. A) Mean of mRNA of Period 1 and Period 2 and dose-dependent influence of IL-1β and TNF (both ng/ml) on MH7A synovial fibroblasts. Effects on CLOCK and BMAL-1 are demonstrated in Additional file 2 Figure S2. The Box Plots are generated from at least 12 independent experiments over 36 hours (n = 19 for IL-1β; n = 12 for TNF). *P < 0.05, #P < 0.005, ##P < 0.001 vs. highest concentration. B) Influence of IL-1β and TNF (both 1 ng/ml) on elapsed time to first BMAL-1 mRNA peak after synchronization. Every symbol represents the time to first peak of one patient during 72 hours of observation (sample interval two hours). *P < 0.05, ##P < 0.001 vs. control (Co).