Table 1 Real time-RT PCR primer sequences
Primer name | Sequences | Probe No. | |
|---|---|---|---|
β-actin | forward | 5'-ATTGGCAATGAGCGGTTC-3' | 11 |
| Â | reverse | 5'-TGAAGGTAGTTTCGTGGATGC-3' | Â |
BMP2 | forward | 5'-CGGACTGCGGTCTCCTAA-3' | 49 |
| Â | reverse | 5'-GGAAGCAGCAACGCTAGAAG-3' | Â |
SOX5 | forward | 5'-TCTGTCCCAGCAGCGTTAG-3' | 41 |
| Â | reverse | 5'-TGACAGCATCATGGTCATTTAAG-3' | Â |
SOX6 | forward | 5'-GCTTCTGGACTCAGCCCTTTA-3' | 50 |
| Â | reverse | 5'-GGCCCTTTAGCCTTTGGTTA-3' | Â |
SOX9 | forward | 5'-GTACCCGCACTTGCACAAC-3' | 61 |
| Â | reverse | 5'-TCGCTCTCGTTCAGAAGTCTC-3' | Â |
TSG6 | forward | 5'-CCAGATGACATCATCAGTACAGG-3' | 78 |
| Â | reverse | 5'-CATTGCAACATATTTGATTTGGA-3' | Â |
STC1 | forward | 5'-CCCAATCACTTCTCCAACAGA-3' | 40 |
| Â | reverse | 5'-TGCTGACTGTGTCTTCATCACA-3' | Â |
COL2A1 | forward | 5'-GTGTCAGGGCCAGGATGT-3' | 75 |
| Â | reverse | 5'-TCCCAGTGTCACAGACACAGAT-3' | Â |
AGGRECAN | forward | 5'-CTGGAAGTCGTGGTGAAAGG-3' | 21 |
| Â | reverse | 5'-TCGAGGGTGTAGCGTGTAGA-3' | Â |