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Figure 4 | Arthritis Research & Therapy

Figure 4

From: CD109, a TGF-β co-receptor, attenuates extracellular matrix production in scleroderma skin fibroblasts

Figure 4

CD109 inhibits extracellular matrix protein production and Smad2/3 phosphorylation in systemic sclerosis and normal fibroblasts. (A, C) Systemic sclerosis (SSc) skin fibroblasts and (B, D) normal skin fibroblasts were transiently transfected with CD109-specific siRNA or control siRNA. Cell lysates were analyzed by western blot using (A, B) anti-CD109 (top panel), anti-fibronectin (second panel), anti-type I collagen (third panel) or anti-CCN2 (fourth panel) antibodies or (C, D) anti-phosphoSmad2, anti-phosphoSmad3, anti-Smad2 or anti-Smad3 antibodies. Membranes were reprobed with an anti-β-actin (bottom panel) antibody to confirm that equal amounts of protein were loaded in each lane. Results presented are representative of three independent experiments. The representative SSc fibroblast data shown correspond to (A) SSc Patient S2 and (C) SSc Patient S16.

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