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Table 1 Effects of anti-TWEAK monoclonal antibodies on the secretion of proinflammatory molecules by dermal fibroblasts stimulated by hTWEAK, alone or in association with TNF or IL-1β

From: Proinflammatory activity of TWEAK on human dermal fibroblasts and synoviocytes: blocking and enhancing effects of anti-TWEAK monoclonal antibodies

   BCB10 BEB3 Stimulation   BCB10 BEB3 Stimulation
Stimulus None (10 μg/ml) (10 μg/ml) index* None (10 μg/ml) (10 μg/ml) index*
  IL-8 secretion (ng/ml)   IL-6 secretion (ng/ml)  
Medium alone 6.4 8.3 5.4 1.0 1.1 1.4 0.9 1.0
hTWEAK (50 ng/ml) 55.1 9.6 96.0 17.8 6.0 2.2 15.9 17.7
TNF (1 ng/ml) 111.6 126.1 114.3 1.0 11.2 11.4 10.8 1.0
TNF + hTWEAK 264.3 136.7 446.1 3.9 21.1 13.9 42.9 4.0
IL-1β (0.1 ng/ml) 214.9 248.6 190.2 1.0 42.5 60.9 53.5 1.0
IL-1β + hTWEAK 439.7 277.2 583.1 3.1 69.8 59.3 116.8 2.2
  MMP-1 secretion (μg/ml)   IP-10 secretion (pg/ml)  
Medium alone 0.44 0.53 0.38 1.0 18.5 nd nd 1.0
hTWEAK (50 ng/ml) 1.68 0.82 2.46 6.5 388 nd 625 33.8
TNF (1 ng/ml) 2.36 2.52 2.49 1.0 241 85 93 1.0
TNF + hTWEAK 2.38 3.09 3.20 1.3 6358 600 6403 68.8
IL-1β (0.1 ng/ml) 2.48 2.82 2.05 1.0 175 103 111 1.0
IL-1β + hTWEAK 3.8 2.89 3.59 1.8 272 83 407 3.7
  PGE2 secretion (ng/ml)   RANTES secretion (ng/ml)  
Medium alone 15 11 10 1.0 0.79 0.63 0.55 1.0
hTWEAK (50 ng/ml) 22 8 31 3.1 10.56 0.77 15.72 28.6
TNF (1 ng/ml) 71 41 57 1.0 2.04 2.65 1.99 1.0
TNF + hTWEAK 125 104 177 3.1 77.88 5.27 91.96 46.2
IL-1β (0.1 ng/ml) 139 156 259 1.0 1.16 1.30 1.49 1.0
IL-1β + hTWEAK 143 186 312 1.2 29.28 1.57 51.78 34.7
  1. * Stimulationindices were calculated from the values obtained with the BEB3 anti-TWEAK mAb results: TWEAK/medium; (TNF + TWEAK)/TNF alone; (IL-1β + TWEAK)/IL-1β alone. Similar results were obtained in 3 independent experiments. hTWEAK, human TNF-like weak inducer of apoptosis; IP-10, interferon-γ-inducible protein-10; MMP-1, matrix metalloproteinase-1; nd, not detectable; PGE2, prostaglandin E2; RANTES, regulated on activation, normal T expressed and secreted; TNF, tumour necrosis factor.