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Table 2 Effects of anti-TWEAK and anti-TNF monoclonal antibodies on the secretion of IL-8, PGE2 and MMP-1 by dermal fibroblasts stimulated by hTWEAK or TNF

From: Proinflammatory activity of TWEAK on human dermal fibroblasts and synoviocytes: blocking and enhancing effects of anti-TWEAK monoclonal antibodies

Stimulus

None

BCB10 (10 μg/ml)

BEB3 (10 μg/ml)

Anti-TNF (10 μg/ml)

  

IL-8 secretion (ng/ml)

 

None

4.9 ± 0.9

5.8 ± 0.6

5.9 ± 0.9

4.6 ± 0.3

hTWEAK (10 ng/ml)

19.8 ± 2.8

6.0 ± 1.2

44.6 ± 6.1

18.8 ± 2.6

TNF(1 ng/ml)

74.3 ± 13

109.3 ± 11

nd

4.5 ± 0.3

  

MMP-1 secretion (ng/ml)

 

None

392 ± 17

494 ± 55

523 ± 68

362 ± 32

hTWEAK (10 ng/ml)

789 ± 35

588 ± 8

1287 ± 69

827 ± 21

TNF(1 ng/ml)

2084 ± 154

2863 ± 141

nd

356 ± 37

  

PGE2 secretion (ng/ml)

 

None

33 ± 2

24 ± 2

90 ± 6

44 ± 14

hTWEAK(50 ng/ml)

47 ± 4

25 ± 2

103 ± 36

52 ± 10

TNF(1 ng/ml)

190 ± 55

212 ± 75

nd

31 ± 5

  

RANTES secretion (ng/ml)

 

None

0.73

0.47

0.72

0.83

hTWEAK(10 ng/ml)

17.22

1.06

47.99

24.96

TNF(1 ng/ml)

1.97

2.20

nd

0.87

  

IP-10 secretion (pg/ml)

 

None

82 ± 10

170 ± 40

nd

157 ± 34

hTWEAK(10 ng/ml)

2032 ± 321

217 ± 75

nd

3274 ± 69

TNF(1 ng/ml)

331 ± 57

253 ± 73

nd

78 ± 42

  1. Values for IL-8, matrix metalloproteinase-1 (MMP-1), prostaglandin E2 (PGE-2) and interferon-γ-inducible protein-10 (IP-10) are shown as mean ± SD; similar results were obtained in 3 independent experiments. Single analyses for RANTES (regulated on activation, normal T expressed and secreted) were done because the amount of available medium was limited. hTWEAK, human TNF-like weak inducer of apoptosis; nd, not done; TNF, tumour necrosis factor.