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Table 2 Effects of anti-TWEAK and anti-TNF monoclonal antibodies on the secretion of IL-8, PGE2 and MMP-1 by dermal fibroblasts stimulated by hTWEAK or TNF

From: Proinflammatory activity of TWEAK on human dermal fibroblasts and synoviocytes: blocking and enhancing effects of anti-TWEAK monoclonal antibodies

Stimulus None BCB10 (10 μg/ml) BEB3 (10 μg/ml) Anti-TNF (10 μg/ml)
   IL-8 secretion (ng/ml)  
None 4.9 ± 0.9 5.8 ± 0.6 5.9 ± 0.9 4.6 ± 0.3
hTWEAK (10 ng/ml) 19.8 ± 2.8 6.0 ± 1.2 44.6 ± 6.1 18.8 ± 2.6
TNF(1 ng/ml) 74.3 ± 13 109.3 ± 11 nd 4.5 ± 0.3
   MMP-1 secretion (ng/ml)  
None 392 ± 17 494 ± 55 523 ± 68 362 ± 32
hTWEAK (10 ng/ml) 789 ± 35 588 ± 8 1287 ± 69 827 ± 21
TNF(1 ng/ml) 2084 ± 154 2863 ± 141 nd 356 ± 37
   PGE2 secretion (ng/ml)  
None 33 ± 2 24 ± 2 90 ± 6 44 ± 14
hTWEAK(50 ng/ml) 47 ± 4 25 ± 2 103 ± 36 52 ± 10
TNF(1 ng/ml) 190 ± 55 212 ± 75 nd 31 ± 5
   RANTES secretion (ng/ml)  
None 0.73 0.47 0.72 0.83
hTWEAK(10 ng/ml) 17.22 1.06 47.99 24.96
TNF(1 ng/ml) 1.97 2.20 nd 0.87
   IP-10 secretion (pg/ml)  
None 82 ± 10 170 ± 40 nd 157 ± 34
hTWEAK(10 ng/ml) 2032 ± 321 217 ± 75 nd 3274 ± 69
TNF(1 ng/ml) 331 ± 57 253 ± 73 nd 78 ± 42
  1. Values for IL-8, matrix metalloproteinase-1 (MMP-1), prostaglandin E2 (PGE-2) and interferon-γ-inducible protein-10 (IP-10) are shown as mean ± SD; similar results were obtained in 3 independent experiments. Single analyses for RANTES (regulated on activation, normal T expressed and secreted) were done because the amount of available medium was limited. hTWEAK, human TNF-like weak inducer of apoptosis; nd, not done; TNF, tumour necrosis factor.