Proinflammatory activity of TWEAK on human dermal fibroblasts and synoviocytes: blocking and enhancing effects of anti-TWEAK monoclonal antibodies

Table 3 Effects of anti-TWEAK and anti-TNF monoclonal antibodies on the secretion of IL-8, PGE₂ and MMP-1 by synoviocytes stimulated by hTWEAK or TNF

Stimulus	None	BCB10 (10 μg/ml)	BEB3 (10 μg/ml)	Anti-TNF (10 μg/ml)
		IL-8 secretion (ng/ml)
None	55.2 ± 3.1	38 ± 1.2	117.6 ± 18	49 ± 14
hTWEAK	146.4 ± 17	73 ± 19	277.2 ± 40	113.8 ± 7.3
TNF	232.6 ± 26	245 ± 33	nd	44.7 ± 4.8
		MMP-1 secretion (ng/ml)
None	4248 ± 276	3848 ± 371	5383 ± 274	3482 ± 584
hTWEAK	6033 ± 773	4381 ± 22	7923 ± 1698	4878 ± 100
TNF	6197 ± 210	7846 ± 713	nd	3505 ± 101
		PGE₂ secretion (ng/ml)
None	50 ± 12	61 ± 6	81 ± 13	47 ± 13
HTWEAK	94 ± 7	87 ± 26	161 ± 30	78 ± 3
TNF	231 ± 49	471 ± 79	nd	53 ± 5
		RANTES secretion (ng/ml)
None	0.12	0.23	0.23	0.05
hTWEAK	30.78	0.26	20.12	26.01
TNF	2.19	1.17	nd	0.05
		IP-10 secretion (pg/ml)
None	274	386 ± 17	349	117 ± 84
hTWEAK	5095 ± 766	330 ± 140	3158	3735 ± 60
TNF	1710 ± 300	1620 ± 20	nd	105

hTWEAK (TNF-like weak inducer of apoptosis) concentration was 10 ng/ml; TNF (tumour necrosis factor) concentration was 1 ng/ml. Values for IL-8, matrix metalloproteinase-1 (MMP-1), prostaglandin E₂ (PGE-2) and interferon-γ-inducible protein-10 (IP-10) are shown as mean ± SD; similar results were obtained in 3 independent experiments. Single analyses for RANTES (regulated on activation, normal T expressed and secreted) were done because the amount of available medium was limited. nd, not done.

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