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Table 3 Effects of anti-TWEAK and anti-TNF monoclonal antibodies on the secretion of IL-8, PGE2 and MMP-1 by synoviocytes stimulated by hTWEAK or TNF

From: Proinflammatory activity of TWEAK on human dermal fibroblasts and synoviocytes: blocking and enhancing effects of anti-TWEAK monoclonal antibodies

Stimulus None BCB10 (10 μg/ml) BEB3 (10 μg/ml) Anti-TNF (10 μg/ml)
   IL-8 secretion (ng/ml)  
None 55.2 ± 3.1 38 ± 1.2 117.6 ± 18 49 ± 14
hTWEAK 146.4 ± 17 73 ± 19 277.2 ± 40 113.8 ± 7.3
TNF 232.6 ± 26 245 ± 33 nd 44.7 ± 4.8
   MMP-1 secretion (ng/ml)  
None 4248 ± 276 3848 ± 371 5383 ± 274 3482 ± 584
hTWEAK 6033 ± 773 4381 ± 22 7923 ± 1698 4878 ± 100
TNF 6197 ± 210 7846 ± 713 nd 3505 ± 101
   PGE2 secretion (ng/ml)  
None 50 ± 12 61 ± 6 81 ± 13 47 ± 13
HTWEAK 94 ± 7 87 ± 26 161 ± 30 78 ± 3
TNF 231 ± 49 471 ± 79 nd 53 ± 5
   RANTES secretion (ng/ml)  
None 0.12 0.23 0.23 0.05
hTWEAK 30.78 0.26 20.12 26.01
TNF 2.19 1.17 nd 0.05
   IP-10 secretion (pg/ml)  
None 274 386 ± 17 349 117 ± 84
hTWEAK 5095 ± 766 330 ± 140 3158 3735 ± 60
TNF 1710 ± 300 1620 ± 20 nd 105
  1. hTWEAK (TNF-like weak inducer of apoptosis) concentration was 10 ng/ml; TNF (tumour necrosis factor) concentration was 1 ng/ml. Values for IL-8, matrix metalloproteinase-1 (MMP-1), prostaglandin E2 (PGE-2) and interferon-γ-inducible protein-10 (IP-10) are shown as mean ± SD; similar results were obtained in 3 independent experiments. Single analyses for RANTES (regulated on activation, normal T expressed and secreted) were done because the amount of available medium was limited. nd, not done.