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Table 1 QPCR of chondrogenic gene expression in cartilage/subchondral bone

From: Hyaluronan injection in murine osteoarthritis prevents TGFbeta 1-induced synovial neovascularization and fibrosis and maintains articular cartilage integrity by a CD44-dependent mechanism

 

acan

P

Fold4

col2a

P

Fold4

col102a

P

Fold4

 

ΔCT1

  

ΔCT1

  

ΔCT1

  

Naive

3.84 (0.30)

 

1.00

4.29 (0.25)

 

1.00

6.33 (0.08)

 

1.00

Acute

2.58 (0.04)

.00012

2.39

1.76 (0.38)

.00012

5.77

6.74 (0.39)

NS2

0.75

TTR

3.59 (0.34)

NS2

1.90

2.82 (0.60)

.00142

2.77

6.59 (0.42)

NS2

0.83

TTR+HA

2.13 (0.24)

.00013

3.27

0.86 (0.19)

.00033

10.7

4.82 (0.30)

.00013

2.85

TTR+SA

3.36 (0.03)

NS3

1.40

2.32 (0. 4)

NS3

3.92

6.16 (0.43)

NS3

1.12

  1. 1relative to GAPDH, mean (SD) ΔCT of 8 pools with 2 mice per pool for Naïve and 12 pools with 2 mice per pool for all 4 treatment groups.
  2. P-values: 2Acute or TTR vs Naive; 3TTR+HA or TTR+SA vs TTR;
  3. 4Fold Changes are relative to Naive
  4. NS, not significant