Hyaluronan injection in murine osteoarthritis prevents TGFbeta 1-induced synovial neovascularization and fibrosis and maintains articular cartilage integrity by a CD44-dependent mechanism

Table 2 QPCR of fibrogenic gene expression in cartilage/subchondral bone

	col1a	P	Fold⁴	col3a	P	Fold⁴	col5a	P	Fold⁴
	ΔCT¹			ΔCT¹			ΔCT¹
Naive	-0.84 (0.23)		1.00	6.67 (0.17)		1.00	9.98 (0.36)		1.00
Acute	-6.03 (0.29)	< .0001²	36.5	5.10 (0.71)	.003²	2.97	8.50 (0.41)	.012²	2.80
TTR	-6.02 (1.03)	< .0001²	36.2	2.95 (0.44)	.0001²	13.2	5.82 (1.02)	.001²	17.9
TTR+HA	-2.44 (0.52)	.0002³	3.03	5.08 (0.59)	.0012³	3.03	7.20 (0.44)	.029³	6.87
TTR+SA	-7.25 (1.84)	NS³	84.4	3.15(1.09)	NS³	11.5	4.87 (0.65)	NS³	34.5

¹relative to GAPDH, mean (SD) ΔCT of 8 pools with 2 mice per pool for Naïve and 12 pools with 2 mice per pool for all 4 treatment groups.
P-values: ²Acute or TTR vs Naive; ³TTR+HA or TTR+SA vs TTR;
⁴Fold Changes are relative to Naive
NS, not significant

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