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Table 1 Primer sequences used in quantitative real-time polymerase chain reaction

From: Decreased hypertrophic differentiation accompanies enhanced matrix formation in co-cultures of outer meniscus cells with bone marrow mesenchymal stromal cells

Gene Primer Direction Reference or GenBank accession number
Beta-actin 5'-AAGCCACCCCACTTCTCTCTAA-3'
5'-AATGCTATCACCTCCCCTGTGT-3'
Forward
Reverse
[36]
Aggrecan 5'-AGGGCGAGTGGAATGATGTT-3'
5'-GGTGGCTGTGCCCTTTTTAC-3'
Forward
Reverse
[36]
Collagen I
(COL1A2)
5'-TTGCCCAAAGTTGTCCTCTTCT-3'
5'-AGCTTCTGTGGAACCATGGAA-3'
Forward
Reverse
[36]
Collagen II
(COL2A1)
5'-CTGCAAAATAAAATCTCGGTGTTCT-3'
5'-GGGCATTTGACTCACACCAGT-3'
Forward
Reverse
[36]
Collagen × (COL10A1) 5'-CAAGGCACCATCTCCAGGAA-3'
5'-AAAGGGTATTTGTGGCAGCATATT-3'
Forward
Reverse
[35]
IHh 5'-CCTTGTCAGCCGTGAGGCCG-3'
5'-GCTGCCGGCTCCGTGTGATT-3'
Forward
Reverse
NM_00218.3
MMP-13 5'-TGGAAGGATGCCTTTTTTTCTC-3'
5'-CACCCTCCCCAAGTATCAATAGG-3'
Forward
Reverse
NM_002427
SOX9 5'-CTTTGGTTTGTGTTCGTGTTTTG-3'
5'-AGAGAAAGAAAAAGGGAAAGGTAAGTTT-3'
Forward
Reverse
[31]
Versican 5'-TGGAATGATGTTCCCTGCAA-3'
5'-AAGGTCTTGGCATTTTCTACAACAG-3'
Forward
Reverse
[35]
  1. All primers were purchased from Invitrogen (Mississauga, ON, Canada). COL1A2, type II collagen α2 chain; COL2A1, type I collagen α1 chain; COL10A1, type × collagen α1 chain; IHh, Indian Hedgehog; MMP-13, matrix metalloproteinase 13; SOX9, Sry-related HMG box 9.