Table 1 Primer sequences used in quantitative real-time polymerase chain reaction
Gene | Primer | Direction | Reference or GenBank accession number |
|---|---|---|---|
Beta-actin | 5'-AAGCCACCCCACTTCTCTCTAA-3' 5'-AATGCTATCACCTCCCCTGTGT-3' | Forward Reverse | [36] |
Aggrecan | 5'-AGGGCGAGTGGAATGATGTT-3' 5'-GGTGGCTGTGCCCTTTTTAC-3' | Forward Reverse | [36] |
Collagen I (COL1A2) | 5'-TTGCCCAAAGTTGTCCTCTTCT-3' 5'-AGCTTCTGTGGAACCATGGAA-3' | Forward Reverse | [36] |
Collagen II (COL2A1) | 5'-CTGCAAAATAAAATCTCGGTGTTCT-3' 5'-GGGCATTTGACTCACACCAGT-3' | Forward Reverse | [36] |
Collagen × (COL10A1) | 5'-CAAGGCACCATCTCCAGGAA-3' 5'-AAAGGGTATTTGTGGCAGCATATT-3' | Forward Reverse | [35] |
IHh | 5'-CCTTGTCAGCCGTGAGGCCG-3' 5'-GCTGCCGGCTCCGTGTGATT-3' | Forward Reverse | NM_00218.3 |
MMP-13 | 5'-TGGAAGGATGCCTTTTTTTCTC-3' 5'-CACCCTCCCCAAGTATCAATAGG-3' | Forward Reverse | NM_002427 |
SOX9 | 5'-CTTTGGTTTGTGTTCGTGTTTTG-3' 5'-AGAGAAAGAAAAAGGGAAAGGTAAGTTT-3' | Forward Reverse | [31] |
Versican | 5'-TGGAATGATGTTCCCTGCAA-3' 5'-AAGGTCTTGGCATTTTCTACAACAG-3' | Forward Reverse | [35] |