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Table 2 Monoclonal antibodies used in the present study

From: Cytokine mRNA and protein expression in primary-culture and repeated-passage synovial fibroblasts from patients with rheumatoid arthritis

Cellular target Antigen Clone Labeling Source
All human cells HLA-ABC W6/32 None DAKO, Hamburg, Germany
Fibroblasts CD90 (Thy-1) FibAS02 None Dianova, Hamburg, Germany
Collagen-producing cells Prolyl-4-hydroxylase 3-2B12 None Calbiochem, Bad Soden, Germany
Antigen-presenting cells (MHC class II) HLA-DP, DQ, DR CR3/43 None DAKO
Monocytes, macrophages CD68 KP1 FITC DAKO
Monocytes CD14 Mφ P9 FITC Becton Dickinson, Heidelberg, Germany
B cells CD19 HD37 None DAKO
T cells CD3 UCHT1 None DAKO
Endothelial cells, some leukocytes CD31 (glycoprotein IIa) JC/70A None DAKO
Endothelial cells von Willebrand's factor 4F9 None Immunotech, Marseilles, France