Table 2 Monoclonal antibodies used in the present study
Cellular target | Antigen | Clone | Labeling | Source |
|---|---|---|---|---|
All human cells | HLA-ABC | W6/32 | None | DAKO, Hamburg, Germany |
Fibroblasts | CD90 (Thy-1) | FibAS02 | None | Dianova, Hamburg, Germany |
Collagen-producing cells | Prolyl-4-hydroxylase | 3-2B12 | None | Calbiochem, Bad Soden, Germany |
Antigen-presenting cells (MHC class II) | HLA-DP, DQ, DR | CR3/43 | None | DAKO |
Monocytes, macrophages | CD68 | KP1 | FITC | DAKO |
Monocytes | CD14 | Mφ P9 | FITC | Becton Dickinson, Heidelberg, Germany |
B cells | CD19 | HD37 | None | DAKO |
T cells | CD3 | UCHT1 | None | DAKO |
Endothelial cells, some leukocytes | CD31 (glycoprotein IIa) | JC/70A | None | DAKO |
Endothelial cells | von Willebrand's factor | 4F9 | None | Immunotech, Marseilles, France |