Table 3 Characterization of synovial and skin fibroblasts by flow cytometry
| Â | Rheumatoid arthritis synovial fibroblasts | Â | |
|---|---|---|---|
Antigen | Primary-culture (n = 4) | Repeated-passage (n = 4) | Primary-culture normal-skin FB (n = 4) |
MHC class I (HLA-ABC) | 98.3 ± 0.8 | 97.6 ± 1.1 | 98.5 ± 0.5 |
Prolyl-4-hydroxylase (intracellular) | 96.5 ± 2.0 | 98.5 ± 0.5 | 98.1 ± 0.3 |
CD90 (Thy-1) | 92.7 ± 4.4 | 98.9 ± 0.3 | 88.8 ± 3.8 |
MHC class II (HLA-DP, DQ, DR) | 3.8 ± 3.1 | 0.3 ± 0.4 | 3.1 ± 2.3 |
CD14 | 0.1 ± 0.1 | 0.2 ± 0.2 | 0.1 ± 0.1 |
CD68 | 0.6 ± 0.7 | 0.2 ± 0.1 | 0.3 ± 0.2 |
CD3 | 1.5 ± 1.0 | 0.3 ± 0.1 | 0.2 ± 0.4 |
CD19 | 0.5 ± 0.9 | 0.1 ± 0.1 | 0.3 ± 0.4 |
von Willebrand's factor (intracellular) | 1.6 ± 1.6 | 0.1 ± 0.1 | 6.0 ± 3.1 |
CD31 | 0.1 ± 0.1 | 0.2 ± 0.1 | 6.1 ± 3.2 |