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Table 3 Characterization of synovial and skin fibroblasts by flow cytometry

From: Cytokine mRNA and protein expression in primary-culture and repeated-passage synovial fibroblasts from patients with rheumatoid arthritis

  Rheumatoid arthritis synovial fibroblasts  
Antigen Primary-culture (n = 4) Repeated-passage (n = 4) Primary-culture normal-skin FB (n = 4)
MHC class I (HLA-ABC) 98.3 ± 0.8 97.6 ± 1.1 98.5 ± 0.5
Prolyl-4-hydroxylase (intracellular) 96.5 ± 2.0 98.5 ± 0.5 98.1 ± 0.3
CD90 (Thy-1) 92.7 ± 4.4 98.9 ± 0.3 88.8 ± 3.8
MHC class II (HLA-DP, DQ, DR) 3.8 ± 3.1 0.3 ± 0.4 3.1 ± 2.3
CD14 0.1 ± 0.1 0.2 ± 0.2 0.1 ± 0.1
CD68 0.6 ± 0.7 0.2 ± 0.1 0.3 ± 0.2
CD3 1.5 ± 1.0 0.3 ± 0.1 0.2 ± 0.4
CD19 0.5 ± 0.9 0.1 ± 0.1 0.3 ± 0.4
von Willebrand's factor (intracellular) 1.6 ± 1.6 0.1 ± 0.1 6.0 ± 3.1
CD31 0.1 ± 0.1 0.2 ± 0.1 6.1 ± 3.2
  1. Values are means ± SD of positive cells. Intracellular, antigens detected following permeabilization of the cells; the remaining antigens were detected by surface staining. FB = fibroblasts.