Table 1 Primer details used for RT-PCR analyses
From: Indian hedgehog gene transfer is a chondrogenic inducer of human mesenchymal stem cells
Gene | RT-PCR primer sequences (5' to 3') | Annealing temperature (°C) | Product size (base pairs) | Cycles |
|---|---|---|---|---|
Chondrogenic markers | ||||
COL type II | Sense: TTTCCCAGGTCAAGATGGTC | 58.0 | 374 | 35 |
Antisense: CTTCAGCACCTGTC CACCA | ||||
AGN | Sense: TGAGGAGGGCTGGAACAAGTACC | 54.0 | 392 | 30 |
Antisense: GGAGGTGGTAATTGCAGGGAACA | ||||
COMP | Sense: CAGGACGACTTTGATGCAGA | 54.0 | 312 | 32 |
Antisense: AAGCTGGAGCTGTCTGGTA | ||||
FMD | Sense: CTTACCCCTATGGGGTGGAT | 54.0 | 389 | 35 |
Antisense: GTACATGGCCGTGAGGAAGT | ||||
SOX9 | Sense: ATCTGAAGAAGGAGAGCGAG | 58.0 | 263 | 35 |
Antisense: TCAGAAGTCTCCAGAGCTTG | ||||
Hypertrophy and osteogenic markers | ||||
COL type X | Sense: CCCTTTTTGCTGCTAGTATCC | 54.0 | 468 | 25 |
Antisense: CTGTTGTCCAGGTTTTCCTGGCAC | ||||
OP | Sense: ACGCCGACCAAGGAAAACTC | 51.0 | 483 | 35 |
Antisense: GTCCATAAACCACACTATCACCTCG | ||||
COL type I | Sense: GGACACAATGGATTGCAAGG | 54.0 | 461 | 32 |
Antisense: TAACCACTGCTCCACTCTGG | ||||
OC | Sense: ATGAGAGCCCTCACACTCCTC | 59.0 | 387 | 35 |
Antisense: GCCGTAGAAGCGCCGATAGGC | ||||
ALP | Sense: TGGAGCTTCAGAAGCTCAACACCA | 51.0 | 454 | 35 |
Antisense: TCTCGTTGTCTGAGTACCAGTCC | ||||
Internal control | ||||
EF-1α | Sense: AGGTGATTATCCTGAACCATCC | 54.0 | 234 | 25 |
Antisense: AAAGGTGGATAGTCTGAGAAGC | ||||