Gene expression profiling and functional analysis of angiogenic markers in murine collagen-induced arthritis

Table 1 Primers applied in this study

Gene	Sense primer5'-3'	Antisense primer 5'-3'	Size (bp)	Reference
Arp	AATCTCCAGAGGCACCATTG	TTCAGCGTGTTCAGCAGTG	101	NM_007475
Flt-1	CGGCAGACCAATACAATCCT	CCGCTGCCTTATAGATGCTC	174	NM_010228
Flk-1/Kdr	CATGCACAGTCTACGCCAAC	CCTCCACGTGTCTCCATTCT	126	NM_010612
Hgf	GGCCATGGTGCTACACTCTT	CTTCTCCTTGGCCTTGAATG	132	NM_010427
Igf-1	TGGATGCTCTTCAGTTCGTG	GCAACACTCATCCACAATGC	113	NM_010512
Il-1β	CAGGCAGGCAGTATCACTCA	ATGAGTCACAGAGGATGGGC	140	NM_008361
Il-6	TACCACTCCCAACAGACCTGTC	CTGCAAGTGCATCATCGTTGTTC	140	NM_031168
Mdk	CCTGCAACTGGAAGAAGGAA	GAGGTGCAGGGCTTAGTCAC	166	NM_010784
Nrp-1	GGAGCTACTGGGCTGTGAAG	CCTCCTGTGAGCTGGAAGTC	134	NM_008737
Plgf	TGCTGGGAACAACTCAACAG	GGACACAGGACGGACTGAAT	146	NM_008827
Tnf	GACCCTCACACTCAGATCATC	CGCTGGCTCAGCCACTCCAGC	105	NM_013693
Vegf120	GGCTGCTGTAACGATGAAGC	GGCTTGTCACATTTTTCTGGC	184	NM_001025257
Vegf164	GGCTGCTGTAACGATGAAGC	GGCTCACAGTGATTTTCTGGC	184	NM_009505
Vefg188	GGCTGCTGTAACGATGAAGC	CTCACAGTGAACGCTCCAG	255	NM_001025250

Sequences represent primer pairs that were designed using Primer3 design package hosted by the Whitehead Institute for Biomedical Research [55]. References present Genbank accession numbers for each gene. All primer pairs were designed to span an intron to exclude potential genomic DNA contamination. (Arp: acidic ribosomal phosphoprotein P0; Flk-1: fetal liver kinase 1; Flt-1: fms-related tyrosine kinase 1; Hgf: hepatocyte growth factor; Igf-1: insulin-like growth factor; Il: interleukin; Mdk: midkine; Plgf: placental growth factor; Vegf: vascular endothelial growth factor)

ISSN: 1478-6362