Table 1 The primer sequences for real-time PCR.
gene | Forward and Reverse Primers |
|---|---|
ALP | 5'-TGGACCTCGTTGACACC-3' 5'-TCCTGTTCAGCTCGTACT -3' |
BSP | 5'-ATACAGGGTTAGCTGCAATC-3' 5'- TCCATTGTCTCCTCCGC -3' |
OCN | 5'-GGCGCTACCTGTATCAATG-3' 5'-AGAGCGACACCCTAGAC -3' |
OSX | 5'-CCACCTACCCATCTGACT-3' 5'-GTTTGGCTCCACCACTC -3' |
Runx2 | 5'-TATTGGTCCTAAGGGAGACATC-3' 5'-TAAAGCGAATGGGCATGTT -3' |
CA1 | 5'-GCTACAGGCTCTTTCAGTT-3' 5'-GACTCCATCCACTGTATGTT -3' |
GAPDH | 5'-ACCACAGTCCATGCCATCAC-3' 5'-TCCACCACCCTGTTGCTGTA -3' |