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Table 1 Primer sequences used for real-time reverse transcription-polymerase chain reaction

From: IL-1ra delivered from poly(lactic-co-glycolic acid) microspheres attenuates IL-1β-mediated degradation of nucleus pulposus in vitro

Primer

Accession number

Direction (5' → 3')

Sequence

Melting temperature, °C

Product length, base pairs

Reference

ADAMTS-4

NM_181667.1

Forward

AACTCGAAGCAATGCACTGGT

54.96

149

[40]

  

Reverse

TGCCCGAAGCCATTGTCTA

53.16

  

GAPDH

NM_001034034.1

Forward

ATCAAGAAGGTGGTGAAGCAGG

54.61

101

[40]

  

Reverse

TGAGTGTCGCTGTTGAAGTCG

55.17

  

INOS

NM_001076799.1

Forward

GTAACAAAGGAGATAGAAACAACAGG

52.30

146

[16]

  

Reverse

CAGCTCCGGGCGTCAAAG

55.52

  

IL-1β

NM_174093.1

Forward

GAGCCTGTCATCTTCGAAACG

59.34

55

This paper

  

Reverse

GCACGGGTGCGTCACA

60.32

  

IL-6

NM_173923.2

Forward

GCTTCACAAGCGCCTTCACTCC

64.47

81

This paper

  

Reverse

GACCCGGGGTAGGGAAAGCAGA

65.73

  

MMP-13

NM_174389.2

Forward

TGGTCCAGGAGATGAAGACC

52.51

80

[40]

  

Reverse

TGGCATCAAGGGATAAGGAA

50.22

  

TLR-4

NM_174198.6

Forward

TGCGTACAGGTTGTTCCTAACATT

54.79

110

This paper

  

Reverse

TAGTTAAAGCTCAGGTCCAGCATCT

55.50

  
  1. ADAMTS-4, a disintegrin and metalloproteinase with thrombospondin motifs 4; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IL-1β, interleukin-1 beta; IL-6, interleukin-6; INOS, inducible nitric oxide synthase; MMP-13, matrix metalloproteinase 13; TLR-4, Toll-like receptor 4.