Protein kinase (PKC)-α/β
is essential for hypoxia-inducible factor alpha (HIF-1α) translocation. (A+B) Detection of HIF-1α, Lamin B and β-actin in nuclear (NF+) and cytosolic (NF-) cell fractions of primary human monocytes using immunoblot. (A) Monocyte protein lysates were acquired after PMA stimulation and incubation for 5 h under hypoxia and under normoxia as indicated. (B) Monocyte protein lysates were acquired after PMA stimulation following PKC-α/β1 inhibition using Gö6976 as indicated and subsequent incubation for 5 h under hypoxia. (A, B) PMA stimulation for 5 h under hypoxia leads to nuclear translocation of HIF-1α, and specific inhibition of PKC-α/β1 by Gö6976 prevents translocation of HIF-1α.