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Table 1 Immunofluorescence patterns and titers of AIT test in SLE-CMV, SLE and CMV groups

From: Response to 'Fragment of tegument protein pp65 of human cytomegalovirus induces autoantibodies in BALB/c mice'

  Titer  
Patterns from AIT test ≤1:20 1:40 1:80 1:160 1:320 1:640 1:1280 ≥1:2560 Total
SLE-CMV groupa          
   Homogeneous       2   9 11
   Homogeneous + speckled       4   13 17
   Homogeneous + cytoplasmic         1 1
   Homogeneous + diffuse granular         4 4
   Homogeneous + speckled + cytoplasmic         3 3
   Speckled        1 8 9
   Speckled + cytoplasmic     1     1 2
   Diffuse granular + cytoplasmic         1 1
   Diffuse cytoplasmic + speckled         3 3
   Diffuse cytoplasmic + diffuse granular         2 2
   Total 0 0 0 1 0 6 1 45 53
SLE groupb          
   Homogeneous       1 2 15 18
   Homogeneous + speckled      2   3 33 38
   Homogeneous + cytoplasmic         2 2
   Homogeneous + diffuse granular        1 12 13
   Homogeneous + speckled + cytoplasmic      2   1 4 7
   Speckled      5 3 5 34 47
   Speckled + cytoplasmic      1 3 3 3 10
   Diffuse granular + cytoplasmic      2   1 5 8
   Diffuse granular       1 5 7 13
   Diffuse cytoplasmic + speckled         9 9
   Diffuse cytoplasmic + diffuse granular       1 1   2
   Diffuse cytoplasmic + nucleolar       1   2 3
   Total 0 0 0 0 12 10 22 126 170
CMV groupc          
   MTOC-MT    4 4 1 1 1   11
   MTOC-MT + IF + speckled   1 1 2    1   5
   Speckled    2       2
   Negative 4         4
   Total 4 1 7 6 1 1 2 0 22
  1. aSLE-CMV group, anti-CMV IgM-positive SLE patients; bSLE group, anti-CMV IgM-negative SLE patients; cCMV group, anti-CMV IgM-positive patients without SLE. The AIT test was performed using an indirect immunofluorescent test kit (IT-AIT™; ImmunoThink Co., Seoul, Republic of Korea). In the CMV group, anti-MTOC-MT-positive patients accounted for 72.7% of the total patients in the CMV group and 88.9% of autoantibody-positive patients, but in the SLE-CMV group, anti-MTOC-MT was not detected at all (P < 0.001). Patients in the SLE-CMV group showed high titers of over 1:640 except for one who received steroid pulse therapy just before the test, but the proportion of patients in the CMV group with a titer over 1:640 was not more than 13.6% (P < 0.001). In addition, most of the autoantibody frequently found in the SLE-CMV group was not found in the CMV group. AIT, autoimmune target; CMV, cytomegalovirus; IF, intermediate filament; MTOC-MT, microtubule organizing center with microtubule; SLE, systemic lupus erythematosus.