Detection of activated fibroblasts in angiotensin II-treated skin. (A) Real-time PCR analysis of αSMA in PBS- and Ang II-treated mice (*P ≤ 0.05). αSMA staining in the upper dermis (B) by immunofluorescence and (C) by immunohistochemistry, and in the lower dermis (D) by immunofluorescence, on sections from PBS- and Ang II-treated mouse skin. Arrows indicate positive staining of myofibroblasts. Representative images are shown from five animals per group.