Figure 6

Recruitment of fibrocytes after angiotensin II treatment. (A) Immunofluorescence staining of CD45 (red) and FSP1 (green) on cryosections of skin samples from PBS- and Ang II-treated mice. Arrows indicate CD45/FSP1 and CD45/P4H double-positive cells. The bar graphs represent quantification of percentage of total positive cells/HPF (high-power field) for CD45, FSP1, CD45/FSP1, and CD45/P4H in PBS- and Ang II-treated mice (*P ≤ 0.05). Representative images are shown from four animals per group. (B) Real-time PCR for MCP1 and FSP1 in PBS (white bars) and Ang II (black bars)-treated mice (*P ≤ 0.05).