Supplementary Figure 5

Production of IFN-γ (a, b, c), IL-4 (d, e, f), and IL-10 (g, h, i) by spleen CD4⁺ T cells after anti-CD4-preincubation. Percentage of cytokine-positive cells (upper panels), their mean fluorescence intensity (MFI) after intracellular staining (middle panels), and secretion of the cytokine into cell culture supernatant as measured by ELISA (lower panels), shown as means ± SEM of three independent experiments (triplicate determinations in pooled cells from three normal rats in each experiment). ^*P ≤ 0.05 in comparison with isotype control, ^#P ≤ 0.05 in comparison with W3/25, ^§P ≤ 0.05 in comparison with OX35, ^&P ≤ 0.05 in comparison with RIB5/2. For key to bars, see Supplementary Figure 4.