Table 1 Proteins identified in the secretome of IL-1β-stimulated chondrocytes with or without CS treatment

MMP2

20.24

17

36.4

[P08253]

72 kDa type IV collagenase

Angiogenesis/collagen degradation

x

A1AT

2

2

6

[P01009]

Alpha-1-antitrypsin

Inhibitor of serine proteases

ANXA2

2

1

36

[P07355]

Annexin A2

Heat-stress response

B2MG

4

3

26.9

[P61769]

Beta-2-microglobulin

Immunity

x

PGS1

4.03

3

13.9

[P21810]

Biglycan

Collagen fiber assembly/ECM component

CATB

3.06

2

15.3

[P07858]

Cathepsin B

Thiol protease

CCL2

6

4

38.4

[P13500]

C-C motif chemokine 2^b

Inflammatory response

CCL8

4

2

38.4

[P80075]

C-C motif chemokine 8^b

Inflammatory response

CH3L1

63.26

96

82

[P36222]

Chitinase-3-like protein 1

ECM component

x

CH3L2

2

2

17.7

[Q15782]

Chitinase-3-like protein 2^b

ECM component

CLUS

10.09

9

43.2

[P10909]

Clusterin

Immunity

x

x

CCD80

2

1

23.3

[Q76M96]

Coiled-coil domain-containing protein 80

Cell adhesion and matrix assembly

CO3A1

2.02

1

14.3

[P02461]

Collagen alpha-1(III) chain

ECM component

CO6A1

4.16

4

28.5

[P12109]

Collagen alpha-1(VI) chain

ECM component

COCA1

17.35

10

21.1

[Q99715]

Collagen alpha-1(XII) chain

ECM component

CO1A2

27.65

25

55.2

[P08123]

Collagen alpha-2(I) chain

ECM component

C1R

8.13

7

31.5

[P00736]

Complement C1r subcomponent

Immunity

x

x

C1S

10.6

9

27.3

[P09871]

Complement C1s subcomponent

Immunity

x

x

CO3

13.4

7

26.4

[P01024]

Complement C3

Immunity

x

CFAB

8.29

6

17.2

[P00751]

Complement factor B

Immunity

x

x

CXCL3

4.01

4

69.2

[P19876]

C-X-C motif chemokine 3^b

inflammatory response

CXCL5

2

1

20.2

[P42830]

C-X-C motif chemokine 5^b

Inflammatory response

CXCL6

4.13

5

38.6

[P80162]

C-X-C motif chemokine 6^b

Inflammatory response

CYTC

4

3

67.1

[P01034]

Cystatin-C

Inhibitor of cysteine proteinases

x

PGS2

23.72

22

56

[P07585]

Decorin

ECM component

DESP

2

1

24.8

[P15924]

Desmoplakin^b

Cell junction

x

FBLN3

15.7

12

31.2

[Q12805]

EGF-containing fibulin-like extracellular matrix protein 1

Negative regulator of chondrocyte differentiation

x

x

FINC

152.34

150

58.9

[P02751]

Fibronectin

ECM component

x

x

FBLN1

2

1

11.2

[P23142]

Fibulin-1

Cell adhesion/ECM organization

x

FSTL1

2.1

2

16.2

[Q12841]

Follistatin-related protein 1

Cell proliferation and differentiation

GDN

24.73

23

54.5

[P07093]

Glia-derived nexin

Serine protease inhibitor

GROA

4

5

54.2

[P09341]

Growth-regulated alpha protein

Inflammatory response

IBP3

12.02

12

57.7

[P17936]

Insulin-like growth factor-binding protein 3

Cell proliferation and differentiation

x

IBP4

3.36

3

37.6

[P22692]

Insulin-like growth factor-binding protein 4

Cell proliferation and differentiation

IBP5

4.03

2

55.5

[P24593]

Insulin-like growth factor-binding protein 5

Cell proliferation and differentiation

IBP6

3.85

3

43.8

[P24592]

Insulin-like growth factor-binding protein 6

Cell proliferation and differentiation

x

IBP7

2.15

3

29.1

[Q16270]

Insulin-like growth factor-binding protein 7

Cell proliferation and differentiation

IL6

16

16

42

[P05231]

IL-6

Inflammatory response

IL8

4.24

6

57.6

[P10145]

IL-8

Inflammatory response

MMP1

35.12

38

50.1

[P03956]

Interstitial collagenase

Collagen degradation

K1C10

60.82

36

66.8

[P13645]

Keratin, type I cytoskeletal 10

Intermediate filament

x

x

K1C14

11.36

7

44.9

[P02533]

Keratin, type I cytoskeletal 14^b

Intermediate filament

K1C16

15.45

8

48

[P08779]

Keratin, type I cytoskeletal 16^b

Intermediate filament

K1C9

30.98

19

60.2

[P35527]

Keratin, type I cytoskeletal 9

Intermediate filament

x

x

K2C1

70.89

41

69.6

[P04264]

Keratin, type II cytoskeletal 1

Intermediate filament

x

x

K22E

43.26

22

69.2

[P35908]

Keratin, type II cytoskeletal 2 epidermal

Intermediate filament

x

x

K2C6B

20.77

11

53.9

[P04259]

Keratin, type II cytoskeletal 6B^b

Intermediate filament

x

MFGM

6.01

4

20.9

[Q08431]

Lactadherin

Angiogenesis

LUM

28.15

30

60.7

[P51884]

Lumican

ECM component

x

x

CSF1

4.01

2

17.3

[P09603]

Macrophage colony-stimulating factor 1^b

Inflammatory response

TIMP1

17.8

16

57.5

[P01033]

Metalloproteinase inhibitor 1

Metalloprotease inhibitor

x

TIMP2

4

3

32.7

[P16035]

Metalloproteinase inhibitor 2

Metalloprotease inhibitor

PTX3

2.02

2

14.4

[P26022]

Pentraxin-related protein PTX3

Inflammatory response

PLTP

5.8

4

31

[P55058]

Phospholipid transfer protein

Lipid transport

x

x

IC1

4

2

18.8

[P05155]

Plasma protease C1 inhibitor

Immunity

x

x

POTEF

2.03

1

27.6

[A5A3E0]

POTE ankyrin domain family member F^b

Unknown

SAP

2

1

5

[P07602]

Proactivator polypeptide^b

Lipid metabolism

PCOC1

5.93

4

19.8

[Q15113]

Procollagen C-endopeptidase enhancer 1

Collagen metabolism

x

S10A8

1.54

1

35.5

[P05109]

Protein S100-A8^b

Inflammatory response

x

S10A9

4

2

34.2

[P06702]

Protein S100-A9^b

Inflammatory response

x

PRG4

3.7

3

21.9

[Q92954]

Proteoglycan 4^b

ECM component

x

x

TRY6

4

2

15.4

[Q8NHM4]

Putative trypsin-6^b

Serine protease

NPT2C

2

1

10.9

[Q8N130]

Sodium-dependent phosphate transport protein 2C^b

Ion transport

SPRC

2

2

7.3

[P09486]

SPARC

Cell proliferation and differentiation

x

MMP3

48.64

47

71.9

[P08254]

Stromelysin-1

Collagen degradation

x

QSOX1

4.87

4

16.3

[O00391]

Sulfhydryl oxidase 1

Cell redox homeostasis

x

SDC4

2

1

13.6

[P31431]

Syndecan-4

ECM component

TARSH

8.86

9

27.4

[Q7Z7G0]

Target of Nesh-SH3

Cell proliferation and differentiation

x

TENA

13.36

8

22.5

[P24821]

Tenascin

Cell adhesion/ECM organization

TETN

4

4

34.2

[P05452]

Tetranectin

Bone mineralization

x

x

TSP1

2.36

2

18.5

[P07996]

Thrombospondin-1

Angiogenesis

x

BGH3

2

1

22.6

[Q15582]

TGF-beta-induced protein ig-h3

TGF-beta signaling

x

TSG6

28.35

30

69.3

[P98066]

TNF-inducible gene 6 protein

Cell adhesion

VCAM1

2

1

12.2

[P19320]

Vascular cell adhesion protein 1

Cell adhesion

x

VASN

2

2

14.7

[Q6EMK4]

Vasorin

TGF-beta signaling

x