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Table 1 Proteins identified in the secretome of IL-1β-stimulated chondrocytes with or without CS treatment

From: Secretome analysis of chondroitin sulfate-treated chondrocytes reveals anti-angiogenic, anti-inflammatory and anti-catabolic properties

Symbol Score Peptides (95%) Covariance (%) Accession numbera Name Function OA serum [22] OA synovial fluid [23]
MMP2 20.24 17 36.4 [P08253] 72 kDa type IV collagenase Angiogenesis/collagen degradation   x
A1AT 2 2 6 [P01009] Alpha-1-antitrypsin Inhibitor of serine proteases   
ANXA2 2 1 36 [P07355] Annexin A2 Heat-stress response   
B2MG 4 3 26.9 [P61769] Beta-2-microglobulin Immunity x  
PGS1 4.03 3 13.9 [P21810] Biglycan Collagen fiber assembly/ECM component   
CATB 3.06 2 15.3 [P07858] Cathepsin B Thiol protease   
CCL2 6 4 38.4 [P13500] C-C motif chemokine 2b Inflammatory response   
CCL8 4 2 38.4 [P80075] C-C motif chemokine 8b Inflammatory response   
CH3L1 63.26 96 82 [P36222] Chitinase-3-like protein 1 ECM component   x
CH3L2 2 2 17.7 [Q15782] Chitinase-3-like protein 2b ECM component   
CLUS 10.09 9 43.2 [P10909] Clusterin Immunity x x
CCD80 2 1 23.3 [Q76M96] Coiled-coil domain-containing protein 80 Cell adhesion and matrix assembly   
CO3A1 2.02 1 14.3 [P02461] Collagen alpha-1(III) chain ECM component   
CO6A1 4.16 4 28.5 [P12109] Collagen alpha-1(VI) chain ECM component   
COCA1 17.35 10 21.1 [Q99715] Collagen alpha-1(XII) chain ECM component   
CO1A2 27.65 25 55.2 [P08123] Collagen alpha-2(I) chain ECM component   
C1R 8.13 7 31.5 [P00736] Complement C1r subcomponent Immunity x x
C1S 10.6 9 27.3 [P09871] Complement C1s subcomponent Immunity x x
CO3 13.4 7 26.4 [P01024] Complement C3 Immunity x  
CFAB 8.29 6 17.2 [P00751] Complement factor B Immunity x x
CXCL3 4.01 4 69.2 [P19876] C-X-C motif chemokine 3b inflammatory response   
CXCL5 2 1 20.2 [P42830] C-X-C motif chemokine 5b Inflammatory response   
CXCL6 4.13 5 38.6 [P80162] C-X-C motif chemokine 6b Inflammatory response   
CYTC 4 3 67.1 [P01034] Cystatin-C Inhibitor of cysteine proteinases x  
PGS2 23.72 22 56 [P07585] Decorin ECM component   
DESP 2 1 24.8 [P15924] Desmoplakinb Cell junction x  
FBLN3 15.7 12 31.2 [Q12805] EGF-containing fibulin-like extracellular matrix protein 1 Negative regulator of chondrocyte differentiation x x
FINC 152.34 150 58.9 [P02751] Fibronectin ECM component x x
FBLN1 2 1 11.2 [P23142] Fibulin-1 Cell adhesion/ECM organization x  
FSTL1 2.1 2 16.2 [Q12841] Follistatin-related protein 1 Cell proliferation and differentiation   
GDN 24.73 23 54.5 [P07093] Glia-derived nexin Serine protease inhibitor   
GROA 4 5 54.2 [P09341] Growth-regulated alpha protein Inflammatory response   
IBP3 12.02 12 57.7 [P17936] Insulin-like growth factor-binding protein 3 Cell proliferation and differentiation x  
IBP4 3.36 3 37.6 [P22692] Insulin-like growth factor-binding protein 4 Cell proliferation and differentiation   
IBP5 4.03 2 55.5 [P24593] Insulin-like growth factor-binding protein 5 Cell proliferation and differentiation   
IBP6 3.85 3 43.8 [P24592] Insulin-like growth factor-binding protein 6 Cell proliferation and differentiation x  
IBP7 2.15 3 29.1 [Q16270] Insulin-like growth factor-binding protein 7 Cell proliferation and differentiation   
IL6 16 16 42 [P05231] IL-6 Inflammatory response   
IL8 4.24 6 57.6 [P10145] IL-8 Inflammatory response   
MMP1 35.12 38 50.1 [P03956] Interstitial collagenase Collagen degradation   
K1C10 60.82 36 66.8 [P13645] Keratin, type I cytoskeletal 10 Intermediate filament x x
K1C14 11.36 7 44.9 [P02533] Keratin, type I cytoskeletal 14b Intermediate filament   
K1C16 15.45 8 48 [P08779] Keratin, type I cytoskeletal 16b Intermediate filament   
K1C9 30.98 19 60.2 [P35527] Keratin, type I cytoskeletal 9 Intermediate filament x x
K2C1 70.89 41 69.6 [P04264] Keratin, type II cytoskeletal 1 Intermediate filament x x
K22E 43.26 22 69.2 [P35908] Keratin, type II cytoskeletal 2 epidermal Intermediate filament x x
K2C6B 20.77 11 53.9 [P04259] Keratin, type II cytoskeletal 6Bb Intermediate filament   x
MFGM 6.01 4 20.9 [Q08431] Lactadherin Angiogenesis   
LUM 28.15 30 60.7 [P51884] Lumican ECM component x x
CSF1 4.01 2 17.3 [P09603] Macrophage colony-stimulating factor 1b Inflammatory response   
TIMP1 17.8 16 57.5 [P01033] Metalloproteinase inhibitor 1 Metalloprotease inhibitor   x
TIMP2 4 3 32.7 [P16035] Metalloproteinase inhibitor 2 Metalloprotease inhibitor   
PTX3 2.02 2 14.4 [P26022] Pentraxin-related protein PTX3 Inflammatory response   
PLTP 5.8 4 31 [P55058] Phospholipid transfer protein Lipid transport x x
IC1 4 2 18.8 [P05155] Plasma protease C1 inhibitor Immunity x x
POTEF 2.03 1 27.6 [A5A3E0] POTE ankyrin domain family member Fb Unknown   
SAP 2 1 5 [P07602] Proactivator polypeptideb Lipid metabolism   
PCOC1 5.93 4 19.8 [Q15113] Procollagen C-endopeptidase enhancer 1 Collagen metabolism   x
S10A8 1.54 1 35.5 [P05109] Protein S100-A8b Inflammatory response x  
S10A9 4 2 34.2 [P06702] Protein S100-A9b Inflammatory response x  
PRG4 3.7 3 21.9 [Q92954] Proteoglycan 4b ECM component x x
TRY6 4 2 15.4 [Q8NHM4] Putative trypsin-6b Serine protease   
NPT2C 2 1 10.9 [Q8N130] Sodium-dependent phosphate transport protein 2Cb Ion transport   
SPRC 2 2 7.3 [P09486] SPARC Cell proliferation and differentiation x  
MMP3 48.64 47 71.9 [P08254] Stromelysin-1 Collagen degradation   x
QSOX1 4.87 4 16.3 [O00391] Sulfhydryl oxidase 1 Cell redox homeostasis x  
SDC4 2 1 13.6 [P31431] Syndecan-4 ECM component   
TARSH 8.86 9 27.4 [Q7Z7G0] Target of Nesh-SH3 Cell proliferation and differentiation x  
TENA 13.36 8 22.5 [P24821] Tenascin Cell adhesion/ECM organization   
TETN 4 4 34.2 [P05452] Tetranectin Bone mineralization x x
TSP1 2.36 2 18.5 [P07996] Thrombospondin-1 Angiogenesis x  
BGH3 2 1 22.6 [Q15582] TGF-beta-induced protein ig-h3 TGF-beta signaling x  
TSG6 28.35 30 69.3 [P98066] TNF-inducible gene 6 protein Cell adhesion   
VCAM1 2 1 12.2 [P19320] Vascular cell adhesion protein 1 Cell adhesion x  
VASN 2 2 14.7 [Q6EMK4] Vasorin TGF-beta signaling x  
  1. Proteins identified by SILAC and liquid chromatography-mass spectrometry analysis in the secretome of IL-1β-stimulated chondrocytes with or without chondroitin sulfate treatment. ECM, extracellular matrix; EGF, epidermal growth factor; TGF, transforming growth factor. aProtein accession number according to the SwissProt and TrEMBL databases. bProtein not identified in our previous analysis of chondrocyte secretome [12].
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Arthritis Research & Therapy

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