Secretome analysis of chondroitin sulfate-treated chondrocytes reveals anti-angiogenic, anti-inflammatory and anti-catabolic properties

Table 2 Extracellular proteins modulated by chondroitin sulfate treatment in IL-1β-stimulated chondrocytes

Accession number^a	Name	Symbol	Ratio^b	Pvalue	Error factor^c
[P00751]	Complement factor B	CFAB	0.5566	0.0064	1.397
[Q08431]	Lactadherin	MFGM	0.597	0.0151	1.3855
[Q7Z7G0]	Target of Nesh-SH3	TARSH	0.7017	0.006	1.2209
[P09871]	Complement C1s subcomponent	C1S	0.7085	0.0007	1.1398
[P01024]	Complement C3	CO3	0.7241	0.0023	1.1999
[P02751]	Fibronectin	FINC	0.7321	0.0006	1.1891
[P03956]	Interstitial collagenase	MMP1	0.7113	0	1.0977
[P00736]	Complement C1r subcomponent	C1R	0.7734	0.0071	1.1755
[P08253]	72 kDa type IV collagenase	MMP2	0.7759	0.0001	1.0999
[P36222]	Chitinase-3-like protein 1	CH3L1	0.8088	0.0216	1.4096
[P10909]	Clusterin	CLUS	0.8224	0.05	1.2162
[P08254]	Stromelysin-1	MMP3	0.8256	0.0086	1.1378
[P07093]	Glia-derived nexin	GDN	1.2894	0.0031	1.1664
[O00391]	Sulfhydryl oxidase 1	QSOX1	1.3064	0.0382	1.2709
[Q92954]	Proteoglycan 4	PRG4	1.4393	0.05	1.445
[P98066]	TNF-inducible gene 6 protein	TSG6	1.7773	0.0007	1.3371
[P61769]	Beta-2-microglobulin	B2MG	2.1652	0.0125	1.4553
[P07996]	Thrombospondin-1	TSP1	89.1678	0	>2

Extracellular proteins identified by SILAC and liquid chromatography-mass spectrometry analysis as modulated by CS treatment in IL-1β-stimulated chondrocytes. ^aProtein accession number according to the SwissProt and TrEMBL databases. ^bAverage SILAC ratios (n = 3) that represent the relative protein abundance in CS-treated versus untreated cells, calculated by Protein Pilot 3.0 software (ABSciex). P ≤ 0.05 was accepted. ^cError factor of the quantification (measure of the error in the average quantification ratio as calculated by Protein Pilot 3.0 software).

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