Figure 3

Human dendritic cells (DCs) are the principal cells implicated in response to smoking. Double immunofluorescence staining of synovial tissue for (A) aryl hydrocarbon receptor (AHR) protein (green) in CMRF44⁺ (red, top panels) or CMRF56⁺ DCs (red, bottom panels). (B) Enlarged images show cytoplasmic and nuclear localization of AHR (green) staining in CMRF44⁺ (red, top panels) or CMRF56⁺ DCs (red, bottom panels) as indicated. (C) CYP1A1 protein (green) in CMRF44⁺ (red, top panels) or CMRF56⁺ DCs (red, bottom panels). (D) AHR and (E), CYP1A1 protein (green) in CD3⁺ T cells (red). In all images, nuclei are counterstained with Hoechst (blue). Scale bar, 20 μm.