Figure 6

Modulation of mitochondrial NADP+-dependent isocitrate dehydrogenase (mNADP-ICDH), type I collagen (Col I) and connective tissue growth factor (CTGF) in isolated adult rat cardiomyocytes. Cells were treated with 10 μM 4-hydroxynonenal (HNE) for 48 hours in the presence or absence of 10 μM ramipril and then total proteins were preceded to the determination of (A) NADP-ICDH activity, (B) HNE/NADP-ICDH adducts, and (C) Col I and CTGF protein as described in Materials and Methods. Values are the mean ± SEM of three experiments. *P < 0.05, ***P < 0.001 (CTL versus HNE), ^#P < 0.05, ^&P < 0.01 (HNE versus HNE+Ram) by one-way ANOVA. CTL, control; Ram, ramipril.