AMP kinase mediates suppression of profibrotic responses by adiponectin. Confluent dermal fibroblasts in two-dimensional monolayer cultures were incubated with adiponectin (5 ug/ml) in media with TGF-β (2 ng/ml) in the presence or absence of Compound C (10 uM) for 48 hours. Total RNA was harvested and subjected to real-time qPCR. The results represent the means ± SEM of triplicate determinations. SEM, standard error of the mean; TGF-β, transforming growth factor-β.