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Table 1 Primers used for real-time qPCR.

From: The adipokine adiponectin has potent anti-fibrotic effects mediated via adenosine monophosphate-activated protein kinase: novel target for fibrosis therapy

Gene Primer sequences Accession number
18S rRNA olg1, 5'-CCCCATGAACGAGGGAATT-3' olg2, 5'-GGGACTTAATCAACGCAAGCTT-3' NR_003286
GAPDH olg3, 5'-CATGAGAAGTATGACAACAGCCT-3' olg4, 5'-AGTCCTTCCACGATACCAAAGT-3' NM_002046
ACTA2 (a-SMA) olg20, 5'-CAGGGCTGTTTTCCCATCCAT-3' olg21, 5'-GCCATGTTCTATCGGGTACTTC-3' NM_001613
COL1A1 olg149, 5'-GCTGGTGTGATGGGATTC-3' olg150, 5'-GGGAACACCTCGCTCT-3' NM_000088
AdipoR1 olg396, 5'-TGACTGGCTAAAGGACAACG-3' olg397, 5'-AAAAGAGAAACAGCACGAAACC-3' NM_015999
AdipoR2 olg400, 5'-CAGCCATTATAGTCTCCCAGTG-3' olg401, 5'-CCGAGATGACATAGTGCAAGG-3' NM_024551
Glut4 olg404, 5'-ACTGGACGAGCAACTTCATC-3' olg405, 5'-GAGGACCGCAAATAGAAGGAA-3' NM_001042
  1. Primer sequences were obtained using Primer Express 3.0 software.
  2. Most primer pairs span two exons, except the intronless 18S rRNA. Primers were tested by dissociation curve analysis to assure only single amplicon.