Figure 1

Histology and T-cell infiltration in lacrimal gland (LG). (A) Total cell infiltration measured in digital images of hematoxylin/eosin (H&E)-stained LG sections of C57BL/6, CD25KO, γDKO, and IFN-γKO mice at ages 8, 12, and 16 weeks (8W,12W,16W, respectively). (B) Representative images of H&E-stained LGs from mice aged 8 to 16 weeks. The blue-dotted circle is a higher magnification of the area demarcated by the black dotted circle in IFN-γKO mice at age 8 weeks. Scale bar, 100 μm. (C) Bar graphs showing mean ± standard deviation of percentage of CD8⁺ T cells gated in LG isolates with flow cytometry in C57BL/6, CD25KO, γDKO, and IFN-γKO mice at ages 8, 12, and 16 weeks. Data are presented as mean ± standard deviation of 10 to 20 individual samples/strain/time point. (D) Representative images of immunohistochemistry for CD8-positive T cells in the LGs of all three strains at different ages. Positive cells are stained red. Scale bar, 50 μm. (E) Bar graphs showing mean ± standard deviation of percentage of CD4⁺ T cells gated in LG isolates with flow cytometry in C57BL/6, CD25KO, γDKO, and IFN-γKO mice at ages 8, 12, and 16 weeks. Data are presented as mean ± standard deviation of 10 to 20 individual samples/strain/time point. (F) Representative images of immunohistochemistry for CD4-positive T cells in the LGs of IFN-γKO, γDKO, and CD25KO mice at ages 8, 12, and 16 weeks. Positive cells are stained red. Scale bar, 100 μm. *P < 0.05; **P < 0.01; ***P < 0.001 intrastrain comparison versus age 8 weeks.