TNF/TNFR signal transduction pathway-mediated anti-apoptosis and anti-inflammatory effects of sodium ferulate on IL-1β-induced rat osteoarthritis chondrocytes in vitro

Table 1 Effects of 1,000 μmol/l sodium ferulate on apoptosis-related gene expression in rat osteoarthritis chondrocytes.

Gene	Model control/normal control (20 ng/ml vs. 0 ng/ml IL-1β)	SF treatment/model control (1,000 μmol/l vs. 0 μmol/l SF in presence of 20 ng/ml IL-1β)
TNF/TNFR pathway
TNF	30.07	0.64
TNFR-1b	3.04	0.39
TRAF-2	2.50	0.43
TRADD	1.98	0.69
Caspase-8	2.30	0.43
Caspase-3	1.88	0.46
NF-κB	2.55	0.56
Other pathways
Fas	1.85	1.00
Fas ligand	0.45	0.42
FADD	0.94	0.58
Bcl-2	0.75	0.59
Bax	0.61	0.66
Bax/Bcl-2	0.80	1.12
Apaf1	0.76	1.03
Caspase 9	0.53	0.48

Each reported value represents the mean fold of mRNA expression relative to the control levels for three biological replicates. FADD, Fas-associated death domain protein; SF, sodium ferulate; TNFR, tumor necrosis factor receptor; TRADD, TNF receptor-associated death domain; TRAF, TNF receptor-associated factor.

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