Figure 2From: Highly efficient genetic transduction of primary human synoviocytes with concentrated retroviral supernatantTransduction of fibroblast-like synovial cells (FLS) with pRET2.EGFP. The FLS from patients with rheumatoid arthritis (RA) were transduced with (a) (d) (g) unconcentrated, (b) (e) (h) 10X concentrated, or (c) (f) (i) 100X concentrated pRET2.EGFP supernatant. (a–c) The percentage of EGFP-positive FLS was determined by flow cytometry. (d–f) Light and (g–i) fluorescence microscopy images of cultures following transduction are shown. These results are representative of data using FLS isolated from 5 RA patients.Back to article page