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Figure 3 | Arthritis Research & Therapy

Figure 3

From: Highly efficient genetic transduction of primary human synoviocytes with concentrated retroviral supernatant

Figure 3

Expression of cyclooxygenase-2 (COX-2) in transduced fibroblast-like synovial cells (FLS). The FLS from patients with rheumatoid arthritis were transduced with retrovirus. Lane 1: 100X concentrated RET2.EGFP; lane 2: 100X concentrated RET2.COX2; lane 3: 10X concentrated RET2.COX2; lane 4: unconcentrated RET2.COX2; lane 5: postcentrifugation supernatant RET2.COX2. Whole cell lysates were analyzed for COX-2 by western blot (lane 6: purified COX-2 protein). The experiment was repeated using FLS lines from different patients with similar results.

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